Effect of small interfering RNA targeting survivin on human bleed urothelial carcinoma cell line T24

Objective Down regulating the expression of survivin mRNA and survivinprotein in the human bleed urothelial carcinoma cell line (T24) to observe its affect to the proliferation and apoptosis of T24 cells.Methods The eukaryotic expression plasmids targeting survivin [psilencer-survivin-small interfering RNA (siRNA),si-Sl,si-S2,si-S3] and negative control plasmid (psilencer-random target sequence-siRNA,si-GL3) were constructed simultaneously,and transfected these plasmids to T24 cells.The expression of survivin mRNA and survivin protein were detected by real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) and Western blotting; the vitality of T24 cells was examined by cell counting kit-8 (CCK-8) assay; the apoptosis of T24 cells were tested by flow cytometry; the Caspase-3 activity was detected by specific colour test.Results In si-S3 treatment,the expression of survivin mRNA and survivin protein decreased significantly 79％ and 80％,cytoactivity declined with depended time and concentration,cell apoptosis increased 20.96％ with increased 85％ Caspase-3 activity,as compared with the control group (every P ＜ 0.05).Conclusion Downregulating survivin could effectively inhibit the proliferation and induce apoptosis of T24cells,which mechanism may be active Caspase-3 in T24 cells. Key words: Small interfere RNA;  survivin;  Human bleed urothelial carcinoma cell line