2006•Science and Technology of Food IndustryRequires access

Determination of pyridoxine by reversed-phase high performance liquid chromatography with chemiluminescence detection

Wang Li, Guangming Zhou, You Shuiying, Xiang Shen

Abstract

基于维生B6对于鲁米诺(Luminol)和高碘酸钾(KIO4)化学发光反应的增敏作用原理,建立了反相高效液相色谱(RP-HPLC)分离柱后化学发光检测VB6的新方法,并成功运用于饮料中VB6的测定.其中在鲁米诺中加入KBr可以大大增敏该反应.该方法测定VB6的线性范围为5.0×10-7～5.0×10-4g/mL,检测限为2 0×10-8g/mL.对1.0×10-5g/mL的VB6进行连续5次测定的相对标准偏差分别为2.8%,回归方程为Y=954.472X+1.488.

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Available abstract

Key concepts: Chemiluminescence, Chromatography, Chemistry, Pyridoxine, High-performance liquid chromatography, Reversed-phase chromatography, Biochemistry