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Research of Enzymatic Hydrolysis of Corn Stalk Pulp with New Cooking Method

He Zhang, Xinjie Tong, Chunsheng Pang, Tujun Xie

Open publisher page 1 citations

Abstract

The objective of this study was to explore technique of enzymatic hydrolysis of corn stalk pulp(removal rate of lignin of 82.1%) from active oxygen cooking with a solid alkali with Chinese cellulase(enzyme activity: 13.94 FPU/100ml).The results showed that the optimum process conditions were the following: enzyme-substrate ratio of 0.47%(m/V),pH value of 4.70,temperature of 50℃ and enzymatic reaction time of 72 h.The highest yield of sugar was 59.41% under the optimum process conditions.The paper also presented the different enzymolysis of corn stalk pretreated by different methods,and the order of the effect of enzymolysis was as follows: corn stalk pulp from active oxygen cooking with a solid base corn stalk pulp from alkaline cooking corn stalk pretreated by mechanical crushing raw corn stalk.

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What this paper is about

The objective of this study was to explore technique of enzymatic hydrolysis of corn stalk pulp(removal rate of lignin of 82.1%) from active oxygen cooking with a solid alkali with Chinese cellulase(enzyme activity: 13.94 FPU/100ml).The results showed that the optimum process conditions were the following: enzyme-substrate ratio of 0.47%(m/V),pH value of 4.70,temperature of 50℃ and enzymatic reaction time of 72 h.The highest yield of sugar was 59.41% under the optimum process conditions.The paper also presented the different enzymolysis of corn stalk pretreated by different methods,and the order of the effect of enzymolysis was as follows: corn stalk pulp from active oxygen cooking with a solid base corn stalk pulp from alkaline cooking corn stalk pretreated by mechanical crushing raw corn stalk.

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Available abstract

The objective of this study was to explore technique of enzymatic hydrolysis of corn stalk pulp(removal rate of lignin of 82.1%) from active oxygen cooking with a solid alkali with Chinese cellulase(enzyme activity: 13.94 FPU/100ml).The results showed that the optimum process conditions were the following: enzyme-substrate ratio of 0.47%(m/V),pH value of 4.70,temperature of 50℃ and enzymatic reaction time of 72 h.The highest yield of sugar was 59.41% under the optimum process conditions.The paper also presented the different enzymolysis of corn stalk pretreated by different methods,and the order of the effect of enzymolysis was as follows: corn stalk pulp from active oxygen cooking with a solid base corn stalk pulp from alkaline cooking corn stalk pretreated by mechanical crushing raw corn stalk.

Key concepts: Stalk, Cellulase, Chemistry, Pulp (tooth), Enzymatic hydrolysis, Hydrolysis, Sugar, Food science

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