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Optimization of enzymatic treatment conditions of corn stalk

Yi JinQiong, HE Yinglong, Xiaojun Su, Keqin Wang

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Abstract

Effects of different factors,such as treating time,temperature,concentrations of substrate and enzyme,on efficiency of enzymatic hydrolysis of corn stalk by cellulose were studied.The orthogonal test with 5 factors and 4 levels was applied.The results showed that significance of factors ordered as follows:reaction temperaturehydrolysis timeconcentration of substrateshaking speedconcentration of enzyme.The optimum conditions for degradation of corn stalk with cellulase were:reaction temperature of 55℃,hydrolysis time of 48h,concentration of substrate of 2%, shaking speed of 130r/min,enzyme concentration 200U/g.The research provided reference for biological utilization of corn stalk.

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Effects of different factors,such as treating time,temperature,concentrations of substrate and enzyme,on efficiency of enzymatic hydrolysis of corn stalk by cellulose were studied.The orthogonal test with 5 factors and 4 levels was applied.The results showed that significance of factors ordered as follows:reaction temperaturehydrolysis timeconcentration of substrateshaking speedconcentration of enzyme.The optimum conditions for degradation of corn stalk with cellulase were:reaction temperature of 55℃,hydrolysis time of 48h,concentration of substrate of 2%, shaking speed of 130r/min,enzyme concentration 200U/g.The research provided reference for biological utilization of corn stalk.

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Available abstract

Effects of different factors,such as treating time,temperature,concentrations of substrate and enzyme,on efficiency of enzymatic hydrolysis of corn stalk by cellulose were studied.The orthogonal test with 5 factors and 4 levels was applied.The results showed that significance of factors ordered as follows:reaction temperaturehydrolysis timeconcentration of substrateshaking speedconcentration of enzyme.The optimum conditions for degradation of corn stalk with cellulase were:reaction temperature of 55℃,hydrolysis time of 48h,concentration of substrate of 2%, shaking speed of 130r/min,enzyme concentration 200U/g.The research provided reference for biological utilization of corn stalk.

Key concepts: Stalk, Cellulase, Cellulose, Substrate (aquarium), Hydrolysis, Enzyme, Chemistry, Enzymatic hydrolysis

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