Study on cellulase enzymatic hydrolysis of microwave-acid pretreated corn stalk.

【Objective】 Pretreated corn stalk leaf,skin and core were investigated by cellulase hydrolysis respectively to confirm the optimal hydrolysis condition of different parts of pretreated corn stalk,and select the most suitable part for the fuel-ethanol.【Method】 The effect of each factor was investigated by single factor experiment and orthogonal experiment,and the result of orthogonal experiment was confirmed by the verification experiment.【Result】 The effect order of the four factors mentioned above to enzymatic hydrolysis of pretreated corn stalk was shown as enzyme-loadtemperaturetime pH.The optimal enzyme-load of the corn stalk core,leaf and skin was 3 000,3 500 and 4 000 U/g,while the optimal temperature was 44,44 and 47 ℃,the optimal time 84,96 and 96 h,and the optimal pH 5.2,5.4 and 5.4 respectively.The sugar content of the hydrolysis under the optimal enzymatic hydrolysis condition of the corn stalk core,leaf and skin was 3.468 7,3.101 6 and 1.828 1 mg/mL.【Conclusion】 The corn stalk cores and leaves are suitable for the fuel-ethanol production,but the corn stalk skin is not the suitable substance for producing fuel ethand.