Effect of early peritoneal drainage on the injury of intestinal mucosa in the rats with severe acute pancreatitis

Objective 　To study the protective effect of early peritoneal drainage against injury of intestinal mucosa in rats with severe acute pancreatitis. Methods 　Thirty-six SD rats were randomly and equally divided into three group, i.e. control group, model (SAP) group, drainage (APD) group. Severe acute pancreatitis was induced in rats by injecting 5% sodium taurocholate (2ml/h, 0.1ml/100g) into the common biliopancreatic duct. All the rats were sacrificed 24 hours after operation. The condition of abdominal cavity was observed, and pathological changes in ileum were detected by HE staining. The serum levels of TNF-α, IL-1β, diamine oxidase (DAO) and D-lactate were determined by ELISA. Results 　Compared with the control group, the histopathological changes in ileum were more marked, and serum levels of TNF-α, IL-1β, DAO and D-lactate were significantly higher in SAP group and APD group (P<0.05); Compared with SAP group, the histopathological changes in ileum were less marked, and serum levels of TNF-α, IL-1β, DAO and D-lactate were significantly lower in APD group (P<0.05). Conclusion 　Early peritoneal drainage can effectively improve the intestinal mucosal injury in acute pancreatitis rats, and reduce the systemic inflammatory response. DOI: 10.11855/j.issn.0577-7402.2016.02.07