Effect of urokinase on intestinal microcirculatory dysfunction in rats with severe acute pancreatitis

Objective:To explore the effect of urokinase on intestinal microcirculatory blood flow and interleukin-1β (IL-1β) in rats with severe acute pancreatitis (SAP). Methods:A total of 192 male Wistar rats were randomly divided into control group,SAP group,and treatment group. The rat model of SAP was established by intravenous injection of 5% sodium taurocholate into the pancreaticobiliary duct. The rats in treatment group were injected with urokinase via the cervical vein. Radioactive biomicrosphere technique was performed to measure the intestinal blood flow after 2,6,12,and 24 hours. The level of IL-1β was detected by radioimmunoassay,and the pathological changes in intestinal mucosa were observed. Results:Compared with control group,in SAP group,the intestinal blood flow significantly decreased (P 0.01) at each time point;the level of IL-1β significantly increased at each time point (P 0.01);and the intestinal mucosal injury was more severe (P 0.01). Compared with SAP group,in treatment group,the intestines blood flow significant increased at 2,6,and 12 hours (P 0.01),but the increase was not significant at 24 hours;the level of IL-1β significantly decreased at each time point (P 0.01);and the pathological changes in intestinal mucosa was significantly improved (P 0.01). Conclusion:In SAP rats,the intestinal blood flow decreases and the level of IL-1β increases. Urokinase could improve intestinal microcirculation and alleviate intestinal injuries in SAP rats.