Immune regulation effect of Ganodermapolysaccharide capsule on immunosuppressed mice

Objective 　To investigate the immune regulation effect of Ganoderma polysaccharide capsule (GPC) on immunosuppressed mice. Methods 　Sixty mice were randomly divided into 6 groups (10 each): normal control group, model group, Lentinan group and 20mg/(kg.d) GPC group, 60mg/(kg.d) GPC group and 180mg/(kg.d) GPC group. The drugs were administrated by intragastric infusion for mice, respectively, once a day for 15 days. From the 13th days, the mice in each group except for control group were injected with cyclophosphamide at a dosage of 80mg/kg, once a day for 3 days. And then the number of peripheral blood cells was counted with veterinary hematology analyzer, the spleen and thymus were weighed and their index were calculated. The T lymphocyte proliferation induced by Con A and LPS was detected with MTT method, NK cytotoxicity was tested with LDH method, carbon clearance was tested by using carbon granules clearance test, and the expressions of IL-2 and IFN-γmRNA in spleen lymphocytes were detected with RT-PCR. Results 　Peripheral blood WBC in 180mg/(kg.d) GPC group, peripheral blood PLT in 60mg/(kg.d) group, the spleen index in 20mg/(kg.d) and 60mg/(kg.d) groups, and thymus index in 20mg/(kg.d) group were increased remarkably when compared with those in model group (P<0.05). Spleen T lymphocyte proliferation induced by Con A and LPS, spleen NK cytotoxicity and carbon clearance in 20mg/(kg.d), 60mg/(kg.d) and 180mg/(kg.d) GPC groups were increased notably when compared with those in the model group (P<0.05 or P<0.01). The expressions of IFN-γmRNA in spleen lymphocytes in 20mg/(kg.d) and 180mg/(kg.d) groups increased markedly when compared with that in the model group (P<0.01). Conclusion 　GPS may enhance immune function and increase peripheral blood WBC and PLT in immunosuppressed mice. DOI: 10.11855/j.issn.0577-7402.2016.09.04