2015•Der pharmacia lettreRequires access

Molecular characterisation of Methicillin resistant Staphylococcus aureus(MRSA) isolated from ocular patients

esh Suresh K, R. Ragunathan, Jesteena Johney

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Abstract

Methicillin-resistant Staphylococcus aureus one of the main pathogen responsible for causing infections of skin and systemic infections. This methicillin-resistant Staphylococcus aureus may be resistant to β-lactam antibiotics as well as other antibiotics. The resistance of Methicillin-resistant Staprhylococci to various was revealed by antibiotic sensitivity test and the gene for antibiotic resistance was revealed by carrying out PCR using mecA and blaIMP primers. The molecular characterization and antibiotic sensitivity test was carried out for the three strains collected from the ocular patients. The MIC test was carried out for all the three strains of Staphylococci using vancomycin which revealed the minimum concentration of vancomycin required to inhibit the growth of this pathogen.

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What this paper is about

Methicillin-resistant Staphylococcus aureus one of the main pathogen responsible for causing infections of skin and systemic infections. This methicillin-resistant Staphylococcus aureus may be resistant to β-lactam antibiotics as well as other antibiotics. The resistance of Methicillin-resistant Staprhylococci to various was revealed by antibiotic sensitivity test and the gene for antibiotic resistance was revealed by carrying out PCR using mecA and blaIMP primers. The molecular characterization and antibiotic sensitivity test was carried out for the three strains collected from the ocular patients. The MIC test was carried out for all the three strains of Staphylococci using vancomycin which revealed the minimum concentration of vancomycin required to inhibit the growth of this pathogen.

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Available abstract

Methicillin-resistant Staphylococcus aureus one of the main pathogen responsible for causing infections of skin and systemic infections. This methicillin-resistant Staphylococcus aureus may be resistant to β-lactam antibiotics as well as other antibiotics. The resistance of Methicillin-resistant Staprhylococci to various was revealed by antibiotic sensitivity test and the gene for antibiotic resistance was revealed by carrying out PCR using mecA and blaIMP primers. The molecular characterization and antibiotic sensitivity test was carried out for the three strains collected from the ocular patients. The MIC test was carried out for all the three strains of Staphylococci using vancomycin which revealed the minimum concentration of vancomycin required to inhibit the growth of this pathogen.

Key concepts: Microbiology, Staphylococcus aureus, Antibiotics, Methicillin-resistant Staphylococcus aureus, Pathogen, Vancomycin, SCCmec, Antibiotic resistance

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