Lactic acid bacteria compositions for application in the meat industry

Despite a large number of the bacterial preparations used in the meat industry, development of new starters and study of their influence on development of undesirable microbiota are still vital. Materials and methods.We studied the halo-and thermoresistance of 8 collection strains of the lactic acid bacteria and the compositions made of these lactic acid bacteria.The antagonistic activity toward the indicator strains, isolated from the raw meat, and the collection bacteria strains was determined by the well-diffusion method.Results and discussion.At the maximum NaCl concentration (10.0%) in the growing medium, the strains of L. plantarum 12 and 1005 were characterized by high growth rate, L. delbrueckii s/sp.lactis 013 and L. casei s/sp.tolerans 290 -by the average one.Thermo-resistant (that is able to grow quickly at the temperature ranged from 5 to 25 °C) were the strains of L. plantarum 12, L. delbrueckii s/sp.lactis013, L. acidophilus 147 and L. casei s/sp.tolerans 187 and 290.The lactic acid bacteria showed the antagonistic activity to both, the strains isolated from the raw meat, and the collection strains of bacteria.Growth of some indicator bacteria was slowed down only, while that of others -completely inhibited.The best antagonists were the strains of L. plantarum 12, L. delbrueckii s/sp.lactis 013 and L. casei s/sp.tolerans 290, which completely inhibited the growth of Bacillus sp. 3, Kurthia sp., Planacoccus sp. 1, sp. 2, Micrococcus sp. 2, Sarcina sp. and Staphylococcus sp., isolated from meat and the collection bacteria, such as: Planacoccus citreus, Escherichia coli and Salmonella enteritidis.Based on the strains of L. plantarum, L. delbrueckii s/ sp.lactis 013 and L. casei s/sp.tolerans 290 nine variants of compositions were made and their biotechnological potential was studied.All the compositions were able to grow even at 0 °C.The most stable was the starter made of L. delbrueckii s/sp.lactis 013 + L. plantarum 12 at the ratio of 1:2, the growth rate of which at 5 °C was estimated as "exceptional".The lactic acid bacteria compositions significantly inhibited the growth of the indicator bacteria.The dimensions of the "no growth' areas for the bacteria isolated from meat ranged from 16 mm to 43 mm depending on the indicator strain and composition.The most sensitive bacteria from the collection ones were the grampositive cocci P. citreus and M. luteus, for which the "no growth" area dimensions, depending on the composition, ranged from 34 mm to 42 mm and from 28 mm to 40 mm, respectively. Conclusions.The results show an increase in the biotech activity of the lactic acid bacteria in the compositions.The most promising for testing in an industrial environment is a composition of L. delbrueckii s/sp.lactis 013 + L. plantarum 12 at the ratio of 1:2.