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Method development and validation of simultaneous determination of Ofloxacin and Satranidazole in pharmaceutical dosage form by RP-HPLC

D. Boopathy, Maid Prakash, Bibin Mathew, Suresh S. Kumar, Paramasivan Thirumalai Perumal

Open publisher page 6 citations

Abstract

A simple, selective, rapid, and precise reverse phase HPLC method has been developed for the simultaneous estimation of Ofloxacin and Satranidazole in pharmaceutical dosage form. A Phenomenex Luna C18 (4.6*250 mm, 5μ) column was used for the separation. The mobile phase was Phosphate buffer: Acetonitrile (70: 30 % v/v) and pH 6.0 at a flow rate of 1.2ml/min with detection at 300nm.The retention time of Ofloxacin and Satranidazole was 3.720and 6.130min, respectively. The developed method was validated in term of accuracy, precision, specificity, system suitability, linearity, and robustness, limit of detection and limit of quantification. The proposed method is suitable for simultaneous determination of Ofloxacin and Satranidazole in pharmaceutical dosage form.

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What this paper is about

A simple, selective, rapid, and precise reverse phase HPLC method has been developed for the simultaneous estimation of Ofloxacin and Satranidazole in pharmaceutical dosage form. A Phenomenex Luna C18 (4.6*250 mm, 5μ) column was used for the separation. The mobile phase was Phosphate buffer: Acetonitrile (70: 30 % v/v) and pH 6.0 at a flow rate of 1.2ml/min with detection at 300nm.The retention time of Ofloxacin and Satranidazole was 3.720and 6.130min, respectively. The developed method was validated in term of accuracy, precision, specificity, system suitability, linearity, and robustness, limit of detection and limit of quantification. The proposed method is suitable for simultaneous determination of Ofloxacin and Satranidazole in pharmaceutical dosage form.

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Available abstract

A simple, selective, rapid, and precise reverse phase HPLC method has been developed for the simultaneous estimation of Ofloxacin and Satranidazole in pharmaceutical dosage form. A Phenomenex Luna C18 (4.6*250 mm, 5μ) column was used for the separation. The mobile phase was Phosphate buffer: Acetonitrile (70: 30 % v/v) and pH 6.0 at a flow rate of 1.2ml/min with detection at 300nm.The retention time of Ofloxacin and Satranidazole was 3.720and 6.130min, respectively. The developed method was validated in term of accuracy, precision, specificity, system suitability, linearity, and robustness, limit of detection and limit of quantification. The proposed method is suitable for simultaneous determination of Ofloxacin and Satranidazole in pharmaceutical dosage form.

Key concepts: Ofloxacin, Dosage form, Chromatography, Chemistry, Detection limit, High-performance liquid chromatography, Phosphate buffered saline, Acetonitrile

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Method development and validation of simultaneous determination of Ofloxacin and Satranidazole in pharmaceutical dosage form by RP-HPLC — Research Paper | ScholarLens