Spectrophotometric and RP-HPLC methods for simultaneous estimation of ofloxacin and satranidazole from tablets

Two simple, accurate and sensitive validatedUVspectrophotometricmethods and one HPLC method for simultaneous estimation of ofloxacin and satranidazole in combined tablet dosage form has been developed. The first developed spectroscopic method employs “area under curve” using 292-296nmand 316-320nmfor estimation of ofloxacin and satranidazole, respectively. The second spectrophotometric method employedwas “Dual wavelengthmethod” using 300.2, 338.8nm for ofloxacin and 276.2, 307.6nm for satranidazole. BeerÂ’s law is obeyed in the concentration range of 2.0 to 20.0i­g/mL-1 and 3.0 to 30i­g/mL-1 of ofloxacin and satranidazole, respectively. DevelopedHPLCmethod is reverse-phase chromatographicmethod using Jasco HPLC systemwith HiQ Sil C18Wcolumn (250i‚´4.6mmi.d.), using acetonitrile: 0.005mol. L-1 tetra butyl ammoniumhydrogen sulphate 70:30 v/v as a mobile phase. The detection was carried out using UV detector set at 320nm. Clonazepamwas used as internal standard. BeerÂ’s law is obeyed in the concentration range of 2.0 to 30.0i­g/mL-1 and 3.0 to 45i­g/mL-1 of ofloxacin and satranidazole, respectively.All the three methods have been successfully applied for the analysis of drugs in pharmaceutical formulations. Results of analysis were validated statistically and by recovery studies.