2010•Zhonghua mazuixue zazhiRequires access
Role of HO-1 in inhibition of oxygen-glucose deprivation-induced apoptosis in rat hippocampal neurons by sevoflurane preconditioning
Jianlin Shao, Xiaohong Wan, Yan Wang, Hang Rong-bi, Heng Xin-hua
Open publisher page 0 citations
Abstract
Objective To investigate the role of HO-1 in inhibition of oxygen-glucose deprivation (OGD)-induced apoptosis in rat hippocampal neurons by sevoflurane preconditioning.Methods Hippoeanlpal neurons of newborn Wistar rats ( 0.05). Compared with group OGD, neuron viability was significantly increased, apoptosis rate was significantly decreased, and expression of HO-1 protein and mRNA was up-regulated in group S1 + OGD and S2 + OGD ( P 0.05 ). Neuron viability was significantly higher, apoptosis rate lower and expression of HO-1 protein and mRNA higher in group S2 + OGD than in group S1 + OGD ( P < 0.01). Neuron viability was significantly lower, apoptosis rate higher and expression of HO-1 protein and mRNA lower in group Z than in group S2+OGD(P<0.01).Conclusion HO-1 is involved in the inhibition of OGD-indueed apoptosis in rat hippocampal neurons by sevoflurane preconditioning.
Key words:
Heme oxygenase (decyclizing) ; Anesthetics, inhalation; Ischemie preconditioning; Reperfusion injury; Neurons; Hippocampns; Apoptosis