2011•Zhongguo xin yao zazhiRequires access

Neuroprotective effect of 3-n-butylphalide on injury induced by oxygen glucose deprivation/reoxygenation in primary neurons from rat hippocampus

YU Yong-cai

Abstract

Objective:To observe neuroprotective effect of 3-n-butylphalide(NBP) on injury induced by oxygen glucose deprivation/reoxygenation(OGD/R),and investigate the possible mechanisms.Methods: Primary hippocampal neurons from newborn SD rats(24h) were cultured in vitro,and injury was induced by OGD/R(30 min/8 h).Neurons were treated with NBP at 0.1,1 and 10μmol·L-1.Apoptotic cells were analyzed by Hoechest33258 staining.Bcl-2 expression was detected by immunocytochemistry.Results: Compared with OGD/R control,NBP significantly decreased neuronal apoptosis(P0.01),and increased the expression of Bcl-2 protein(P0.01) in a concentration-dependent manner.Conclusion: NBP inhibits OGD/R-induced apoptosis of hippocampal neurons and protecte the neurons.This effect may be via increasing apoptosis-inhibited Bcl-2 protein expression.

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Key concepts: Neuroprotection, Hippocampal formation, Apoptosis, Immunocytochemistry, Hippocampus, Chemistry, Protein expression, Pharmacology