2008•Chongqing yixueRequires access
Effect of hemin on expression of heme oxygenase-1 in hippocampal organotypic tissue cultures after combined oxygen/glucose deprivation
Gang Yu
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Abstract
Objective To investigate the neuroprotective role of hemin and its effect on the expression of heme oxygenase-1(HO-1) in the hippocampal organotypic tissue cultures(HOTC) after combined oxygen/glucose deprivation(OGD).Methods SD rats,8-10d old,were sacrificed for brain slices.HOTC were made and randomly divided into four groups:HOTC,HOTC undergoing OGD(OGD),HOTC undergoing OGD following pretreatment with hemin(Hemin+OGD);HOTC undergoing OGD following pretreatment with Znpp(Znpp+OGD).The expression of HO-1 was detected by immunohistochemical technique and computer-assisted image analysis.Results HO-1 expression was observed in the neurons and gliacytes in HOTC group.In OGD group,hippocampal organotypic tissue lost normal structure and neurons were damaged severely.Pretreatment with hemin could effectively reverse the damage of the neurons,and pretreatment with Znpp,a HO-1 inhibitor,could aggravate the damage.The expression of HO-1 protein in the Hemin+OGD group was significantly stronger than that in the OGD group.The expression of HO-1 in Znpp+OGD group was significantly weaker than that in the OGD group.Conclusion Hemin has the protective effect on injury induced by OGD in rat hippaocampal slices,which is associated with enhanced expression of HO-1 in neurons and gliacytes.