2008•Unpublished venueRequires access
Effects of 1,25-dlhydroxyvitamin D《,3》 on the cell cycle and apoptosis of K562 leukemia cells
Zuo Wenli, Xue Yu-xian, Yongping Song, Yufu Li
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Abstract
Objective To investigate effects of 1,25-Dihydroxyvitamin D3 on the cell cycle and apoptosis of K652 cells. Methods Vitamin D receptor (VDR) expression was identified by western blot analysis. Cell growth, apoptosis and cell cycle were evaluated by MTT assay, acridine orange/ethidium bromide (AO/EB) and flow cytometry. Results VDR was present in K562 cell nucleus. 10-8mol/L 1, 25-Dihydroxyvitamin D3 could markedly inhibit K562 cells growth and induced cell apoptosis. Most of cells were arrested in G2/M phase, the ratio of apoptosis increased from 4.1% in control group to 26.5 % in treatment group (P<0.01). Conclusion 1,25-Dihydrexyvitamin D3 exhibited obvious inhibitory effects on cell proliferation and cell cycle, and induced apoptosis in K562 cells.
Key words:
Calcitriol; Leukemia, myeloid, chronic; Cell cycle; Apoptosis