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[Effects of an antioxidant (An7845) on the proliferation and apoptosis of K562 leukemia cells].

Kui Yan Song, Qun He

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Abstract

OBJECTIVE: To determine the effects of an antioxidant (An7845) on the proliferation inhibition and apoptosis induction of a human leukemia cell line (K562). METHODS: We evaluated the effects of An7845 on K562 cell proliferation by cellular enumeration in liquid culture assay, MTT assay and colony formation assay. We also verified the effects of An7845 on K562 cell apoptosis by cellular morphology and the DNA fragments appeared in agarose gel electrophoresis. RESULTS: The proliferation of K562 leukemia cells was inhibited by An7845 (5 x 10(-8) - 5 x 10(5) mol/L) in a dose-dependent manner. K562 cells were induced toward apoptosis by An7845 (5 x 10(-7) mol/L). CONCLUSION: An7845 can effectively inhibit the proliferation and induce the apoptosis of K562 leukemia cells.

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What this paper is about

OBJECTIVE: To determine the effects of an antioxidant (An7845) on the proliferation inhibition and apoptosis induction of a human leukemia cell line (K562). METHODS: We evaluated the effects of An7845 on K562 cell proliferation by cellular enumeration in liquid culture assay, MTT assay and colony formation assay. We also verified the effects of An7845 on K562 cell apoptosis by cellular morphology and the DNA fragments appeared in agarose gel electrophoresis. RESULTS: The proliferation of K562 leukemia cells was inhibited by An7845 (5 x 10(-8) - 5 x 10(5) mol/L) in a dose-dependent manner. K562 cells were induced toward apoptosis by An7845 (5 x 10(-7) mol/L). CONCLUSION: An7845 can effectively inhibit the proliferation and induce the apoptosis of K562 leukemia cells.

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Available abstract

OBJECTIVE: To determine the effects of an antioxidant (An7845) on the proliferation inhibition and apoptosis induction of a human leukemia cell line (K562). METHODS: We evaluated the effects of An7845 on K562 cell proliferation by cellular enumeration in liquid culture assay, MTT assay and colony formation assay. We also verified the effects of An7845 on K562 cell apoptosis by cellular morphology and the DNA fragments appeared in agarose gel electrophoresis. RESULTS: The proliferation of K562 leukemia cells was inhibited by An7845 (5 x 10(-8) - 5 x 10(5) mol/L) in a dose-dependent manner. K562 cells were induced toward apoptosis by An7845 (5 x 10(-7) mol/L). CONCLUSION: An7845 can effectively inhibit the proliferation and induce the apoptosis of K562 leukemia cells.

Key concepts: K562 cells, Apoptosis, Leukemia, Agarose gel electrophoresis, Cell growth, MTT assay, Molecular biology, Cell culture

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[Effects of an antioxidant (An7845) on the proliferation and apoptosis of K562 leukemia cells]. — Research Paper | ScholarLens