Immunohistochemistry in 169 Cases of Pituitary Adenoma

Aims and ObjectivesTo study the morphological and immunohistochemical profile of Pituitary Adenomas in relation to determination of cell differentiation & classification, clinical features, radiological features and biochemical data. JMSCR Vol||05||Issue||01||Page 15884-15901||January 2017Histology Tissue sections from formalin-fixed Paraffin-Embedded blocks were stained by Haematoxylin and Eosin and observed by Light Microscopy.Immunohistochemistry 4micrometre thick paraffin sections were collected on Triaminopropylethoxysilane-coated glass slides and subjected to Immunohistochemistry using the Streptavidin-Biotin Complex Immunoperoxidase method, with the following Antibodies:Positive Controls were employed for the Antibodies as below.S. No. Antibody Positive Control 1. CK Colonic Mucosa 2. FSH Pituitary, K/c/o Gonadotroph Adenoma 3. PRL Pituitary, K/c/o Prolactinoma 4. HGH Normal Pituitary 5. TSH Normal Pituitary 6. LH Normal Pituitary 7. ACTH Normal PituitaryImmunostaining was evaluated in the fields consisting of regions of the tumour having the greatest number of immunoreactive cells, as assessed qualitatively at low-power examination.Cytoplasmic staining, in > 10% of cells was regarded as positive.