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FREE RADICAL SCAVEGIG ACTIVITY OF AQUEOUS EXTRACT OF SPHAERATHUS IDICUS (LI).

S. Prabhu, Richard Lobo, Annie Shirwaikar

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Abstract

Summary The free radical scavenging potential of the plant Sphaeranthus indicus Linn. was studied by using different antioxidant models of screening. The aqueous extract at 1000 g/ ml showed maximum scavenging of the radical cation, 2,21 azinobis1 (3 1 ethylbenzothiazoline1 61 sulphonate) (ABTS) observed upto 26.21 % followed by the scavenging of the stable radical 1,11 diphenyl, 21 picryl hydrazyl (DPPH) (24.95 %), superoxide dismutase (17.74 %) and nitric oxide radical (10.63 %) at the same concentration. However, the extract showed only moderate scavenging activity of iron chelation (9.62 %). Total antioxidant capacity of the extract was found to be 127. 85 nmol/g ascorbic acid. The findings justify the therapeutic applications of the plant in the indigenous system of medicine, augmenting its therapeutic value.

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Summary The free radical scavenging potential of the plant Sphaeranthus indicus Linn. was studied by using different antioxidant models of screening. The aqueous extract at 1000 g/ ml showed maximum scavenging of the radical cation, 2,21 azinobis1 (3 1 ethylbenzothiazoline1 61 sulphonate) (ABTS) observed upto 26.21 % followed by the scavenging of the stable radical 1,11 diphenyl, 21 picryl hydrazyl (DPPH) (24.95 %), superoxide dismutase (17.74 %) and nitric oxide radical (10.63 %) at the same concentration. However, the extract showed only moderate scavenging activity of iron chelation (9.62 %). Total antioxidant capacity of the extract was found to be 127. 85 nmol/g ascorbic acid. The findings justify the therapeutic applications of the plant in the indigenous system of medicine, augmenting its therapeutic value.

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Available abstract

Summary The free radical scavenging potential of the plant Sphaeranthus indicus Linn. was studied by using different antioxidant models of screening. The aqueous extract at 1000 g/ ml showed maximum scavenging of the radical cation, 2,21 azinobis1 (3 1 ethylbenzothiazoline1 61 sulphonate) (ABTS) observed upto 26.21 % followed by the scavenging of the stable radical 1,11 diphenyl, 21 picryl hydrazyl (DPPH) (24.95 %), superoxide dismutase (17.74 %) and nitric oxide radical (10.63 %) at the same concentration. However, the extract showed only moderate scavenging activity of iron chelation (9.62 %). Total antioxidant capacity of the extract was found to be 127. 85 nmol/g ascorbic acid. The findings justify the therapeutic applications of the plant in the indigenous system of medicine, augmenting its therapeutic value.

Key concepts: Chemistry, DPPH, Antioxidant, Scavenging, Ascorbic acid, ABTS, Aqueous solution, Traditional medicine

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