2006•PubMedRequires access

[Determination of istanbulin A in Sarcandra glabra].

Ai-hua Zeng, Yongming Luo, Ning Liu

Abstract

OBJECTIVE: To determine istanbulin A in Sarcandra glabra (Thunb.) Nakai. METHODS: An RP-HPLC method was established and an hypersil ODS C18 column (4.6 mm x 200 mm, 5 microm) was used. The detective wavelength was at 218nm. RESULTS: Istanbulin A had a good linearity in the range of 0. 135 to approximately 2. 16 microg. The recovery rate was 98.54%. CONCLUSION: This method is simple, accurate and repeatable. It can be used to determine istanbulin A in Sarcandra glabra.

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Key concepts: Chromatography, Recovery rate, Chemistry