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[Determination of isofraxidin in Sarcandra glabra (Thunb.) Nakai by HPLC].

Gui-Fen Zhou, Honglin Liu, Honghuei Wang, Panyong Kuang

Open publisher page 2 citations

Abstract

OBJECTIVE: To establish a method for the determination of isofraxidin in Sarcandra glabra. METHOD: Using HPLC with mu-Bondapak C18 column, acetonitrile -0.1% phosphoric acid solution(20:80) as mobile phase and detection wavelength at 344 nm. RESULT: The recovery and RSD were 98.1% and 1.9% (n = 5) respectively. CONCLUSION: This method is rapid, simple and accurate, thus may be used for the quality control of this drug.

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What this paper is about

OBJECTIVE: To establish a method for the determination of isofraxidin in Sarcandra glabra. METHOD: Using HPLC with mu-Bondapak C18 column, acetonitrile -0.1% phosphoric acid solution(20:80) as mobile phase and detection wavelength at 344 nm. RESULT: The recovery and RSD were 98.1% and 1.9% (n = 5) respectively. CONCLUSION: This method is rapid, simple and accurate, thus may be used for the quality control of this drug.

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Available abstract

OBJECTIVE: To establish a method for the determination of isofraxidin in Sarcandra glabra. METHOD: Using HPLC with mu-Bondapak C18 column, acetonitrile -0.1% phosphoric acid solution(20:80) as mobile phase and detection wavelength at 344 nm. RESULT: The recovery and RSD were 98.1% and 1.9% (n = 5) respectively. CONCLUSION: This method is rapid, simple and accurate, thus may be used for the quality control of this drug.

Key concepts: Phosphoric acid, Chromatography, High-performance liquid chromatography, Chemistry, Organic chemistry

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