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[Effects of celecoxib on PGE2 synthesis and COX-2 and VEGF-C mRNA expression in Tca8113 cell lines].

Weizhong Li, Xiaoyan Wang, Yanqing Ding

Open publisher page 1 citations

Abstract

OBJECTIVE: To investigate the effect of the selective cyclooxygenase-2 (COX-2) inhibitor celecoxib on prostaglandin E(2) (PGE2) release and vascular endothelial growth factor C (VEGF-C) and COX-2 mRNA expression in Tca8113 cell lines. METHODS: MTT assay was used to analyze the proliferation of Tca8113 cells. The PGE2 level was detected with enzyme-linked immunosorbent assay (ELISA), and the expressions of COX-2 and VEGF-C mRNA were examined with RT-PCR. RESULTS: Celecoxib could induce inhibitory effects on the growth and PGE2 release in Tca8113 cells. The RT-PCR results showed that celecoxib significantly down-regulated the expression of VEGF-C mRNA, but produced a weak effect on COX-2 mRNA expression. CONCLUSION: The inhibitory effect of celecoxib on Tca8113 cell growth and the expressions of VEGF-C and COX-2 may be related to the release of PGE2.

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OBJECTIVE: To investigate the effect of the selective cyclooxygenase-2 (COX-2) inhibitor celecoxib on prostaglandin E(2) (PGE2) release and vascular endothelial growth factor C (VEGF-C) and COX-2 mRNA expression in Tca8113 cell lines. METHODS: MTT assay was used to analyze the proliferation of Tca8113 cells. The PGE2 level was detected with enzyme-linked immunosorbent assay (ELISA), and the expressions of COX-2 and VEGF-C mRNA were examined with RT-PCR. RESULTS: Celecoxib could induce inhibitory effects on the growth and PGE2 release in Tca8113 cells. The RT-PCR results showed that celecoxib significantly down-regulated the expression of VEGF-C mRNA, but produced a weak effect on COX-2 mRNA expression. CONCLUSION: The inhibitory effect of celecoxib on Tca8113 cell growth and the expressions of VEGF-C and COX-2 may be related to the release of PGE2.

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Available abstract

OBJECTIVE: To investigate the effect of the selective cyclooxygenase-2 (COX-2) inhibitor celecoxib on prostaglandin E(2) (PGE2) release and vascular endothelial growth factor C (VEGF-C) and COX-2 mRNA expression in Tca8113 cell lines. METHODS: MTT assay was used to analyze the proliferation of Tca8113 cells. The PGE2 level was detected with enzyme-linked immunosorbent assay (ELISA), and the expressions of COX-2 and VEGF-C mRNA were examined with RT-PCR. RESULTS: Celecoxib could induce inhibitory effects on the growth and PGE2 release in Tca8113 cells. The RT-PCR results showed that celecoxib significantly down-regulated the expression of VEGF-C mRNA, but produced a weak effect on COX-2 mRNA expression. CONCLUSION: The inhibitory effect of celecoxib on Tca8113 cell growth and the expressions of VEGF-C and COX-2 may be related to the release of PGE2.

Key concepts: Celecoxib, Messenger RNA, Prostaglandin E2, Vascular endothelial growth factor, Cell growth, MTT assay, Cyclooxygenase, Chemistry

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[Effects of celecoxib on PGE2 synthesis and COX-2 and VEGF-C mRNA expression in Tca8113 cell lines]. — Research Paper | ScholarLens