Effects of celecoxib on the expression of PGE2 and VEGF A549 cell lines

Objective To investigate the effect of the selective cyclooxygenase-2 inhibitor celecoxib on the expression of PGE2 and VEGF induced by IL-1β in A549 cell lines.Methods A549 cells were incubated with IL-1β in the presence or absence of celecoxib.After indicated incubation time,cells were harvested to detect VEGFmRNA by reverse transcription-polymerase chain rection(RT-PCR),the medium was collected to determine the concentration of PGE2 and VEGF by enzyme-linked immunosorbent assay(ELISA).Results After Celecoxib intervention,the formation of PGE2 and VEGF expression decreased more than control group(IL-1β induced),the difference being significance(P0.05);with the concentration of Celecoxib stepping up,the formation of PGE2 and VEGF expression was decreased in dose-dependent manner,and the difference within different groups was significant(P0.05),the percent rate of decrease in the formation of PGE2 and VEGF expression being almost the same.Conclusion These results suggest celecoxib down-regulats interleukin-1β induced VEGF expression in dose-dependent manner in A549 cells,and the mechanism may be carried out by inhibiting PGE2 formation.