Effect of IL-1β on MMP-3 expression in human trabecular cells

Objective To investigate the effect of Interleukin 1β(IL-1β)on the expression of matrix metalloproteinases(MMPs)in cultured human trabecular cells(HTCs).Methods Human trabecular cells were primarily cultured and subcultured.The 3rd passage cells were incubated with different dosages of IL-1β(0、5、10 、25、50 ng/ml,final concentration,diluted by DMEM/F12 without serum)for 24 hours.MMP-3 were determined by RT-PCR and ELISA.Results The cultured HTCs expressed MMP-3.RT-PCR detected that The mean gray scale of MMP-3 /GAPDH expressed by the HTCs treated with 0 ng/ml(control)、5 ng/ml、10 ng/ml、25 ng/ml、50 ng/ml IL-1βfor 24h was0.1037±0.0494、0.3437±0.0764、0.8588±0.0443、 1.0079±0.0347、1.3466±0.0309,respectively,The difference between these 5 ng/ml、10ng/ml、25 ng/ml、50ng/ml treated group and that of the control group was statistically significant.ELISA detected that The constration of MMP-3 expressed by the HTCs treated with 0 ng/ml(control)、5 ng/ml、10ng/ml、25ng/ml、50ng/ml IL-1βfor 24h was1.2437±0.3910 ng/ml、2.0325±0.2456 ng/ml、2.4607±0.3207 ng/ml、2.8532±0.1392 ng/ml、3.5858±0.1141 ng/ml,respectively,The difference between these 5 ng/ml、10ng/ml、25ng/ml、50ng/ml treated group and that of the control group was statistically significant.Conclusion In certain extent,IL-1β can incease expression of MMP-3 in cultured HTCs and increase the degradation of extracellular matrix(ECM)localized in trabecular meshwork and decrease the resistance of aqueous outflow,followed by IOP reduction.