[Study on isozymes of farm cultivars of Fructus trichosanthis from Shandong].

OBJECTIVE: To determine different cultivars of Fructus trichosanthis of Shandong, and explore their genetic relationships. METHODS: The peroxidase isozyme and esterase isozyme of cultivars and wild plant were studied by means of polyarcylamide gel electrophresis, and the finger-prints were established. RESULTS: The zymogram of the peroxidase isozyme and the esterase isozyme of different cultivars and wild plant of Frustus trichosanthis consisted of common bands, but they had more or less difference in the number of isozymic bands, their positions and activity intensity. CONCLUSION: There are closer genetic relationships between Niuxin Gualou with Ren Gualou, and Xiao Guang-dan with wild sample; Mingpi and Xiao Guang-dan are two different cultivars; The isozyme analysis can provide reliable basis for determining different cultivars of Fructus trichosanthis of Shandong.