The Isozyme Analysis of Some Soybean Varieties from Heilongjiang Province

Polyacrylamide gel electrophoresis(PAGE)was adopted to study Peroxidase isozyme,Esterase isozyme and Superoxide dismutase of 25 soybean varieties from Heilongjiang province.The results showed that zymograms of these POD,EST,SOD of 25 soybean varieties were similarly but with some differences in terms of band number,mobility and isozymes activity(enzyme with depth).A total of 23 bands were detected in 25 cultivars using three isozymes markers,the polymorphic bands were 13,amount to 56.52%,statistical results revealed that the similarity coefficient among the 25 varieties ranged from 0.56 to 0.95,and the average was 0.76.It suggested that there were genetic variations among the varieties.According to the cluster analysis,the tested materials could be divided into 5 groups at the level GS 0.8.