Determination of Ephedrine Hydrochloride in Xiaoerkechuanling Granules by Two Different HPLC Methods:A Contrastive Analysis

OBJECTIVE:To determine the content of Ephedrine hydrochloride in Xiaoerkechuanling granules by two HPLC methods.METHODS:The chromatographic conditions for the first HPLC method were as follows:the phenomenex C18 column(250 mm×4.6 mm,5 μm) was used with methanol-water(1∶1) as mobile phase at a detection wavelength of 254 nm;the chromatographic conditions for the second HPLC method were as follows:the phenomenex C18 column(250 mm×4.6 mm,5 μm)was used with acetonitrile-phosphoric acid solution(contain 0.02 mol·L-1 potassium dihydrogen phosphate and 0.01 mol·L-1 phosphoric acid)(5∶95) as mobile phase at a detection wavelength of 210 nm.RESULTS:By HPLC method one,the linear range of Ephedrine hydrochloride was 0.08 μg·mL-1～40 μg·mL-1(r=0.999 9)with the average recovery at 101.72% and the RSD at 2.77%;by the second HPLC method,the linear range of Ephedrine hydrochloride was 0.4 μg·mL-1～400 μg·mL-1(r=1.000 0) with the average recovery at 99.60% and the RSD at 2.91%.CONCLUSION:Both of the two HPLC methods are accurate,specific and reproducible,but by the first HPLC method,the extraction was more complete and more operable,and no organic solvent was used.