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Shoot and Plantlet Formation in Organ and Callus Cultures of Albizzia lebbek Benth

Sudha Upadhyaya, N. Chandra

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Abstract

Plantlets were produced in vitro from root and hypocotyl explants taken from seedlings of the tree legume, Albizzia lebbek. These explants formed shoots when cultured with 5.0 mg l−1 kinetin and 1.0 mg l−1 IAA in MS medium. Shoots were also induced in large numbers from callus treated with benzylaminopurine. About 20 per cent of the shoots rooted and were grown into plants.

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What this paper is about

Plantlets were produced in vitro from root and hypocotyl explants taken from seedlings of the tree legume, Albizzia lebbek. These explants formed shoots when cultured with 5.0 mg l−1 kinetin and 1.0 mg l−1 IAA in MS medium. Shoots were also induced in large numbers from callus treated with benzylaminopurine. About 20 per cent of the shoots rooted and were grown into plants.

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Available abstract

Plantlets were produced in vitro from root and hypocotyl explants taken from seedlings of the tree legume, Albizzia lebbek. These explants formed shoots when cultured with 5.0 mg l−1 kinetin and 1.0 mg l−1 IAA in MS medium. Shoots were also induced in large numbers from callus treated with benzylaminopurine. About 20 per cent of the shoots rooted and were grown into plants.

Key concepts: Biology, Callus, Shoot, Plantlet, Kinetin, Explant culture, Hypocotyl, Botany

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