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Plantlet Regeneration from Hypocotyls in vitro Culture of Dichondra repens Forst

Tian Zhi

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Abstract

The method of plantlet regeneration from hypocotyls in vitro culture of Dichondra repens Forst. was developed. The hypocotyls could grow quickly aseptically from seed germination in darkness, then a mass of calli could be induced from hypocotyls in vitro culture on MS medium supplemented with 0.2 mg/L 2,4-D and 0.5 mg/L 6-BA, the calli were transferred to B5 medium supplemented with 2 mg/L 6-BA and 0.2 mg/L α-NAA for regenerating.

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What this paper is about

The method of plantlet regeneration from hypocotyls in vitro culture of Dichondra repens Forst. was developed. The hypocotyls could grow quickly aseptically from seed germination in darkness, then a mass of calli could be induced from hypocotyls in vitro culture on MS medium supplemented with 0.2 mg/L 2,4-D and 0.5 mg/L 6-BA, the calli were transferred to B5 medium supplemented with 2 mg/L 6-BA and 0.2 mg/L α-NAA for regenerating.

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Available abstract

The method of plantlet regeneration from hypocotyls in vitro culture of Dichondra repens Forst. was developed. The hypocotyls could grow quickly aseptically from seed germination in darkness, then a mass of calli could be induced from hypocotyls in vitro culture on MS medium supplemented with 0.2 mg/L 2,4-D and 0.5 mg/L 6-BA, the calli were transferred to B5 medium supplemented with 2 mg/L 6-BA and 0.2 mg/L α-NAA for regenerating.

Key concepts: Hypocotyl, Plantlet, Germination, In vitro, Botany, Regeneration (biology), Biology, Repens

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