2003•PubMedRequires access
[High-dose glucose induces human retinal endothelial cell apoptosis].
Jianfang Ma, Zhonghan Yang, Zhihong Song, Yin Guo, Weibin Cai, Qian-ping Liu, Linlang Guo, Guoquan Gao
Open publisher page 1 citations
Abstract
OBJECTIVE: To examine the direct effect of high glucose levels on primary cultured human retinal capillary endothelial cells (HRCEC). METHODS: HRCECs were isolated from donated eyes and cultured for 6 days in the media containing 5 or 25 mmol/L glucose. The cell viability was determined by trypan blue exclusion assay and cell cycle analyzed by flow cytometry, with the cell apoptosis assayed by TUNEL method. RESULTS: The cell viability was significantly decreased after exposure to 25 mmol/L glucose, and the number of apoptotic cells determined by flow cytometry and TUNEL was significantly increased in response to high-dose glucose treatment. CONCLUSION: High-dose glucose induces apoptosis in HRCEC, which may contribute to the development of diabetic retinopathy.