2011•Basic & Clinical MedicineRequires access
Overexrpession of miR-16 promotes apoptosis in Neuro2a cells
Chang Liu, Wei Liu, Ming Wang, Bin Yin, Yuan Jian-Gang
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Abstract
Objective To investigate the role of miR-16 in cell cycle and apoptosis in Neuro2a cells and explore the possible function of miR-16 in tumor.Methods The overexpression plasmid pcDNA3.1-miR-16 of miR-16 was cloned and introduced into Neuro2a cells.Real-time PCR was chosen to test the overexpression of miR-16.Cell cytometry was used to study the role of miR-16 in cell cycle and apoptosis,and apoptosis cells were quantitatively examined by TUNEL.Results After tranfection of pcDNA3.1-miR-16,the mature miR-16 was significantly overexpressed(P0.05).Cell cytometry results showed that the cell cycle didn't change after miR-16 overexpression while cell apoptosis were found and confirmed by TUNEL.Conclusion There was significant apoptosis of Neuro2a cell after overexpression of miR-16,the result suggested miR-16 may have potential significane in treatment of tumor.