ScholarLens Back to search
2001•Zhonghua wei-chang waike zazhiRequires access

Experiment on apoptosis of human gastric adenocarcinoma cell(MGC-803) induced by introduction of wild-type P53gene

Yalin Liu

Open publisher page 0 citations

Abstract

Objective To explore the inhibiting mechanism of proliferation of human gastric adenocarcinoma cell(MGC-803)by wild-type P53gene.Methods A P53gene recombinant adenovirus vector,AdCMVP53,was constructed a nd transfected into the cultured MGC-803.The cell cycle and apoptosis rate were analysed with flow cytometry.Apoptosis was determi ned by termined deoxynucleotidyl transferase-mediated d-UTP nick en d labeling(TUNEL)and agarose gel electrophoresis.Results Introduction of wild-type P53into M GC-803arrested 75.7%of AdCMVP53-infected cells at G 0 /G 1 phase of cycle,leading to inhibition of DNA s ynthesis.The apoptosis rate was 20.8%.Electrophoresis of genomic DNA showed internucleosomal fragme nt of DNA isolated from the AdCMVP53-infected MGC-803.TUNEL and flow cytometry revealed apoptosis of AdCMVP53-infected cells.Conclusion Wild-type P53gene can suppress MGC-803proliferation by i nduction of apoptosis.

About this research paper

What this paper is about

Objective To explore the inhibiting mechanism of proliferation of human gastric adenocarcinoma cell(MGC-803)by wild-type P53gene.Methods A P53gene recombinant adenovirus vector,AdCMVP53,was constructed a nd transfected into the cultured MGC-803.The cell cycle and apoptosis rate were analysed with flow cytometry.Apoptosis was determi ned by termined deoxynucleotidyl transferase-mediated d-UTP nick en d labeling(TUNEL)and agarose gel electrophoresis.Results Introduction of wild-type P53into M GC-803arrested 75.7%of AdCMVP53-infected cells at G 0 /G 1 phase of cycle,leading to inhibition of DNA s ynthesis.The apoptosis rate was 20.8%.Electrophoresis of genomic DNA showed internucleosomal fragme nt of DNA isolated from the AdCMVP53-infected MGC-803.TUNEL and flow cytometry revealed apoptosis of AdCMVP53-infected cells.Conclusion Wild-type P53gene can suppress MGC-803proliferation by i nduction of apoptosis.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To explore the inhibiting mechanism of proliferation of human gastric adenocarcinoma cell(MGC-803)by wild-type P53gene.Methods A P53gene recombinant adenovirus vector,AdCMVP53,was constructed a nd transfected into the cultured MGC-803.The cell cycle and apoptosis rate were analysed with flow cytometry.Apoptosis was determi ned by termined deoxynucleotidyl transferase-mediated d-UTP nick en d labeling(TUNEL)and agarose gel electrophoresis.Results Introduction of wild-type P53into M GC-803arrested 75.7%of AdCMVP53-infected cells at G 0 /G 1 phase of cycle,leading to inhibition of DNA s ynthesis.The apoptosis rate was 20.8%.Electrophoresis of genomic DNA showed internucleosomal fragme nt of DNA isolated from the AdCMVP53-infected MGC-803.TUNEL and flow cytometry revealed apoptosis of AdCMVP53-infected cells.Conclusion Wild-type P53gene can suppress MGC-803proliferation by i nduction of apoptosis.

Key concepts: TUNEL assay, Apoptosis, Agarose gel electrophoresis, Molecular biology, Flow cytometry, Cell cycle, Terminal deoxynucleotidyl transferase, Transfection

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Experiment on apoptosis of human gastric adenocarcinoma cell(MGC-803) induced by introduction of wild-type P53gene — Research Paper | ScholarLens