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[Expression of AML1/ETO9a isoform in acute myeloid leukemia-M2 subtype].

Yuqing Miao, Zixing Chen, Jun He, Jiannong Cen, Xiaojing Bao, Qiao‐Cheng Qiu, Dong‐Er Zhang, Ming Yan

Abstract

OBJECTIVE: To investigate the expression of AML1/ETO9a isoform in the acute myeloid leukemia (AML)-M2 patients. METHODS: Expressions of AML1/ETO fusion gene and AML1/ETO9a isoform were detected by using reverse transcriptase-polymerase chain reaction (RT-PCR) in leukemia patients, MDS patients, leukemia cell lines and healthy subjects. Karyotype was studied by R-banding technique. RESULT: In 30 newly diagnosed AML-M2 patients 15 were found to express AML1/ETO9a isoform, while the rest including 20 AML-M2CR, 18 other subtypes of AML, 5 chronic myelogenous leukemia (CML), 3 myelodysplastic syndromes (MDS), 3 leukemia cell lines (NB4, KG-1, K562) and 5 healthy subjects were AML1/ETO9a negative. Among the 15 AML/ETO9a isoform expressing cases, 13 were demonstrated t(8;21) translocation and AML1/ETO expression. CONCLUSION: Isoform AML1/ETO9a was correlated to AML/M2, and it may promote the development of leukemia in combination with the AML1/ETO fusion gene.

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Key concepts: Myeloid leukemia, Gene isoform, Leukemia, Chromosomal translocation, Fusion gene, Cancer research, Myeloid, Polymerase chain reaction