2007•PubMedRequires access

[Inhibiting E7 oncogene in CaSki cells of cervical cancer by vector-based RNA interference technique].

Danqing Wang, Zhilan Peng, Xiaoyu Niu, Da-ke Li

Abstract

OBJECTIVE: To test of the effect of vector-based RNA interference (RNAi) technique on inhibiting HPV16E7 gene in CaSki cells of cervical cancer. METHODS: The HPV16E7-specific siRNA expression vectors P1, P2 and P3 were constructed and transfected into CaSki cells by liposome. The expression of HPV16E7 mRNA and protein were detected by real-time RT-PCR and Western blot. RESULTS: The expression of HPV16E7 mRNA and protein decreased with the transfection of P1, P2 and P3. Vector P1 had the strongest inhibition effect, with an inhibition rates of 92.86% and 81.0% for the expression of HPV16E7 mRNA and protein respectively three weeks after transfection. CONCLUSION: The expression of E7 gene in CaSki cells can be inhibited by HPV16E7-specific siRNA expression vector.

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Key concepts: Transfection, RNA interference, Molecular biology, Messenger RNA, Oncogene, Expression vector, Vector (molecular biology), Western blot