RNA interference of the the TES gene expression and tumor cell proliferation

Objective To observe impact of RNA interference of the TES gene expression and tumor cell proliferation.Methods Small interfering RNA (siRNA) specific-targeting TES gene was transfected into endometrial cancer cell line Ishikawa,studying its effects on TES expression with quantitative Real-time polymerase chain reaction (Real-time PCR) and western blotting,cellular proliferation was determined by methyl thiazol tetrazolium (MTT) assay.Results At different time point (12,24,48,72 h),the TES mRNA expression in experimental group were 0.675,0.024,0.324,0.916,significantly lower compared with control (P ＜ 0.05) ; there was no statistically significant difference between the control groups (P ＞ 0.05).MRNA level of TES reached the lowest at 24 h in the experimental group.TES protein in Ishikawa cells was consistent with TES mRNA levels change after RNA interference:12-48 h after transfection,the experimental group TES protein expression were 65.2,3.2,25.6,33.0,was significantly reduced,and the differences were statistically significant (P ＜ 0.05) ; TES protein expression was also the lowest at 24 h.After transfection with siRNA,the proliferation activity of Ishikawa cells was enormously enhanced (P ＜ 0.05).Conclusion After RNA interference,TES mRNA and protein levels showed a significant downward trend,proliferation of Ishikawa cells also was significantly enhanced,confirmed that TES gene functioned as a tumor suppressor in endometrial cancer. Key words: Tumor;  Tumor suppressor gene ;  RNA interference