Suppressive effect of RNA interference on BCSG-1 gene expression in MCF-7 cell line

Objective To investigate the suppressive effects of siRNA on the BCSG-1 gene expression in breast cancer MCF-7 cells. Methods The four-pair designed and chemically synthesized in vitro of small interfering RNA (siRNA) sequences ( BCSG-1-siRNA-1 ,-2,-3 and 4) for BCSG-1, and one pair of non-specific siRNA sequence were transducted into plasmid vectors, following by transfection into into MCF-7 cells. Blank control group (empty vector transfection) and normal control group (no transfection) were set up. After 48 h, the mRNA and protein expression levels of BCSG-1 in transfected cells were detected by using reverse transcription-polymerase chain reaction (RT-PCR) and immunocytochemistry respectively. Results The expression of BCSG1 protein was 4.27 ±0. 12, 4.19 ±0.22, 4.15 ± 0.14,4.17 ±0.13, 7.92 ±0.22, 8.02 ±0.13, 8.02 ±0.13, and that of BCSG1 mRNA was 0.624 ±0.010,0.626±0.013, 0.634 ±0.008, 0.631 ±0.010, 0.976±0.076, 0.983 ±0.052, 1.014±0.034 respectively. As compared with control groups, there was significant difference in the expression of BCSG-1 mRNA and protein among the above groups (P ＜0.01 ). The expression level of BCSG-1 mRNA and protein in the groups of MFC-1 cells transfected with BCSG-1-siRNA was lower than that in control groups.Conclusion Expression of BCSG-1 gene can be effectively inhibited by siRNA in MCF7 cells. Key words: BCSG1;  RNA-interference;  Transfection;  Inhibition