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Comparison of restriction endonuclease cleavage patterns in salmonid herpesvirus DNAs.

Lin Ja

Abstract

Herpesvirus salmonis (H. salmonis) and seven strains of Oncorhynchus masou virus (OMV) were analyzed for their genomes with restriction endonucleases HindIII and PstI. Restriction profiles of H. salmonis were different from those of OMV strains. Restriction patterns of OMV strain DNAs were divided into four subtypes with HindIII. With PstI digestion, DNA restriction patterns of those strains were divided into five subtypes. Obvious difference in restriction patterns was not observed among tumor tissue-derived and coelomic fluids-derived strains. By using 32P-labelled DNA of standard OMV (strain 00-7812) as a probe, H. salmonis DNA was not hybridized, but most of the fragments of OMV strain DNAs were hybridized.

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Key concepts: PstI, Restriction enzyme, HindIII, Biology, Restriction fragment, DNA, Restriction map, Genome