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Restriction site maps of the human adenovirus type 8 DNA.

Mária Takács, G Berencsi, Anna Lengyel, I Nász

Abstract

Restriction site maps of human adenovirus 8 (Ad h 8) were constructed with BamHI, HindIII, PstI, SalI and KpnI endonucleases. The genome size was found to be 22.1 to 22.3 X 10(6) Mr. Comparison of the results with the data available on h Ad subgenera A, B, C showed that the SalI enzyme revealed subgenus-specific differences in the genomes. Similar patterns of the SalI fragments in both type 8 and 10 suggest that the differences were specific for the subgenus D of h Ad.

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Key concepts: PstI, HindIII, BamHI, Subgenus, Restriction enzyme, Restriction site, Restriction map, Biology