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[Cloning and prokaryotic expression of heat shock protein 70 gene of Mycobacterium tuberculosis].

Jing Ye, Yan-Fang Sui, Guangsheng Chen, Xiumin Zhang

Open publisher page 3 citations

Abstract

AIM: To clone the heat shock protein 70 (Hsp70) gene of Mycobacterium tuberculosis and express it in E.coli. METHODS: Hsp70 gene was amplified by PCR from Mycobacterium tuberculosis H37Rv, then inserted into pUC19 vector and sequenced. The recombinant expression plasmid pGEX-TBhsp70 was constructed and expressed in E.coli. RESULTS: Hsp70 gene was obtained form Mycobacterium tuberculosis and its sequence was identical with that reported in GenBank. The E.coli. containing pGEX-TBhsp70 could express a protein with M(r) being 96,000 after induction of IPTG. CONCLUSION: A confirmed Hsp70 gene is cloned and expressed in E.coli successfully, which lay the foundation for the related research of the gene.

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What this paper is about

AIM: To clone the heat shock protein 70 (Hsp70) gene of Mycobacterium tuberculosis and express it in E.coli. METHODS: Hsp70 gene was amplified by PCR from Mycobacterium tuberculosis H37Rv, then inserted into pUC19 vector and sequenced. The recombinant expression plasmid pGEX-TBhsp70 was constructed and expressed in E.coli. RESULTS: Hsp70 gene was obtained form Mycobacterium tuberculosis and its sequence was identical with that reported in GenBank. The E.coli. containing pGEX-TBhsp70 could express a protein with M(r) being 96,000 after induction of IPTG. CONCLUSION: A confirmed Hsp70 gene is cloned and expressed in E.coli successfully, which lay the foundation for the related research of the gene.

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Available abstract

AIM: To clone the heat shock protein 70 (Hsp70) gene of Mycobacterium tuberculosis and express it in E.coli. METHODS: Hsp70 gene was amplified by PCR from Mycobacterium tuberculosis H37Rv, then inserted into pUC19 vector and sequenced. The recombinant expression plasmid pGEX-TBhsp70 was constructed and expressed in E.coli. RESULTS: Hsp70 gene was obtained form Mycobacterium tuberculosis and its sequence was identical with that reported in GenBank. The E.coli. containing pGEX-TBhsp70 could express a protein with M(r) being 96,000 after induction of IPTG. CONCLUSION: A confirmed Hsp70 gene is cloned and expressed in E.coli successfully, which lay the foundation for the related research of the gene.

Key concepts: pUC19, Mycobacterium tuberculosis, Biology, Gene, Molecular biology, Plasmid, Heat shock protein, Recombinant DNA

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[Cloning and prokaryotic expression of heat shock protein 70 gene of Mycobacterium tuberculosis]. — Research Paper | ScholarLens