Cloning and Prokaryotic Expression of Rv1884c Gene of Mycobacterium tuberculosis

Objective:To construct fusion gene and prokaryotic expression plasmid of the Rv1884c gene of Mycobacterium tuberculosis,and to express the fusion proteins efficiently in Escherichia coli.Methods:The Rv1884c gene was amplified by PCR with specific primers from genomic DNA of M.tuberculosis H37Rv strain,and was cloned into pGEX-4T-1 expression vector.After sequenced,DH5α strain of E.coli was transformed with the recombinant vector and induced to express recombinant proteins.The relative moleculer size of the proteins were analyzed by SDS-PAGE.Results:The length of PCR products was 531 bp and identica1 with what the GenBank reported.The recombinant expressive vector pGEX-4T-1-Rv1884c was constructed.The E.coli DH5α strains with recombinant vector showed high level of Rv1884c gene expressions after IPTG induction.Conclusion:The fusion Rv1884c gene and prokaryotic expression plasmid were constructed.The expression of recombinant Rv1884c protein with natural activity lays a basis for further study of fast cultivation in M.tuberculosis.