Cloning and expression of the Mycobacterium tuberculosisgene protein Hsp16.3

The aim was to clone and express the gene coding for Mycobacterium tuberculosis antigen Hsp16.3,and to establish a basis for the further research of biology and immunology.The gene coding for Hsp16.3 was amplified by polymerase chain reaction(PCR),and then the gene was inserted to vector pET30a to construct the recombinant plasmid.The recombinant plasmid was transformed into expressive vector E.coli BL21(DE3) and was induced with IPTG.The presence of recombinant protein in the expression vector was analyzed by SDS-PAGE.The results indicated that the recombinant plasmid with the correct target gene was constructed,and the recombinant protein was expressed in E.coli BL21(DE3).In conclusion the target gene was cloned into the host bacterium and expressed correctly,and these results would establish the basis for the further research in biology and immunology of Hsp16.3.