2007•Journal of Shenyang Medical CollegeRequires access

Effect of CGRP and NGF on the Expression of PKB during Whole Cerebral Ischemia and Reperfusion in Rats

Xing Xue

Abstract

Objective:To investigate the expression of protein kinase B(PKB) in hippocampus after regional cerebral ischemia and reperfusion in rats,explore the protective effects and mechanism of calcitonin and gene-related peptide(CGRP) and nerve growth factor(NGF) on brain tissue.Methods:An novel model of regional cerebral ischemia and reperfusion induced by middle cerebral artery occluded (MCAO)in rats was mode.The expression of PKB in hippocampus and cortex was detected with electron microscope,SABC immunohistochemical method and analyzed by microimage system.Results:The expression of PKB in hippocampus and cortex was increased after cerebral ischemia and reperfusion(P0.05).Compared with this,the percentage of PKB immunoreaction positive cells in CGRP or NGF group were higher(P0.01).The combined usage of CGRP and NGF enhanced such effects(P0.05).Conclusion:The results indicate that CGRP and NGF participate in the regulation of expression of PKB in ischemic neurons,and they may cooperate with each other.

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Key concepts: Calcitonin gene-related peptide, Ischemia, Nerve growth factor, Internal medicine, Endocrinology, Hippocampus, Medicine, Calcitonin