Rat hyperlipidemia model set up using lipid mulsion

Objective To establish a hyperlipemia rat model using lipid emulsion,choose the best hyperlipemia models for preventing and healing hyperlipoidemia.Methods Thirty wistar rats(20 male and 10 female),twenty male wistar rats were divided into two groups by weight randomly,these two groups were the second and the third group in our experiment.Ten female wistar rats were the third group.The first group fed by lipid emulsion which included 10% cholesterol.The second and the third group fed by lipid emulsion which included 20% cholesterol.The serum total cholesterol(TC),triglyeride(TG),high densitv lipoprotein(HDL) and low densitv lipoprotein(LDL) were tested at the zero day and fourteenth day respectively.Results(1) The rats fed by lipid emulsion which included 10% cholesterol at fourteen day,the serum TC,HDL and LDL were higher than the incipient level.The rats fed by lipid emulsion which included 20% cholesterol,the serum TC,HDL,TG and LDL of the female and male wistar rats were higher and with statiatical significance.(2) In the case of the same sex and different method,the difference of the serum TC and LDL have statiatical significance;With the second method,the different sex.The difference of the serum TG and HDL have statiatical significance.Conclusion Establishing a hyperlipemia rat model successfully by using lipid emulsion,when the male rats were fed by lipid emulsion which included 20% cholesterol had obvious effects,that were suitable for the research of mixed hyperlipoidemia,hypercholesteremia and hypertriglyceridemia.