Preparation of brusine liposomes

AIM: To study the influences of surfactant concentration, cholesterol concentration, the pH value of solution, preparation temperature, and drug concentration on the particle size and encapsulation efficiency of brusine nano-liposome. METHODS: The nano-liposomes was prepared by film-ultrasonic wave technology and the drug contents in liposomes were determined by gel chromatography-visible light chromatometry. RESULTS: By film-ultrasonic wave dissolving techniques, the large unilamellar liposome in the range from 50 nm to 300 nm was prepared. The optimum preparation technique was HPC and CHL in w/w ration of 3∶1, Tween-80 and total liposome in w/w ratio of 3∶10, at 40(°C) reactive temperature, 1 mg/mL drug concentration and solution pH7.4. The prepared brusine nano-liposomes demonstrated good decentralization and encapsulation efficiency high to 61.24%. CONCLUSION: Film-ultrasonic wave is a simple method to fabricate nano-brusine liposome.