Preparation of Breviscapine Liposome and Determination of Its Physicolchemical Properties

AIM: To prepare the breviscapine liposome and to determine its physicolchemical properties METHOD: Liposomes were prepared by film evaporation method,treated further by lyophilization The concentration of liposome was detected by RP HPLC and the encapsulation efficiency was determined by UV spectrophotometric method after liposome was dialyzed The particle size and Zeta potential were determined by Zetamaster The cumulative release percentage of liposome suspension was tested in 0 9%NaCl RESULT: The liposome loading amount was 20 1%±0 88%( n =3) and the encapsulation efficiency was 81 1%±1 1%( n =3) The mean particle size and Zeta potential was 50nm±12nm( n =3) and 24mV±9mV( n =3) respectively The average cumulative release percentage of liposome in 0 9%NaCl was 17 2%,26 1%,and 29 9% in 2,8 and 24 hours respectively CONCLUSION: The loading and encapsulation efficiency of breviscapine liposome were both good,and the mean particle size was in the scope of nanometer,so that the long circulating and targeting drug delivery system could be realized