Preparation of Docetaxel Liposome and Investigation on Its Stability

OBJECTIVE: To prepare docetaxel liposome and to investigate its stability. METHODS: Docetaxel liposome was prepared with thin- film ultrasonic dispersion technology; the content of the principal agent was determined by HPLC; the indices such as grain size, Zeta potential, encapsulation efficiency, and its stability under the airtight storage for 2 weeks or stored at 4℃ for 6 months were investigated. RESULTS: The prepared liposome was small in grain size and well- distributed. The grain size, Zeta potential, and encapsulation efficiency of liposome were( 138. 8± 1. 1) nm, ( 2. 25± 0. 2) mV and above 70% , respectively. The linear range of docetaxel was 0. 032 5～ 2. 600mg· mL- 1( r=0. 999 3) , with average recovery at 100. 91% ( RSD=1. 38% , n=3) . No obvious changes were found in all the indices in the the stability of the liposome. CONCLUSION: The preparation was proved to be of high encapsulation efficiency and good stability.