Effects of resveratrol on proliferation and apoptosis of nasopharyngeal carcinoma cell line CNE-2Z

Object To investigate the effects of resveratrol (Res) on the proliferation and apoptosis of nasopharyngeal carcinoma cell line CNE-2Z. Methods IC 50 values were detected by MTT assay. The apoptosis was analyzed by flow cytometry (FCM), Hoechst 33258/PI fluorescence staining, and DNA agarose gel electrophoresis. Results After CNE-2Z cells were treated with different concentrations of Res for 24, 48, and 72 h, their IC 50 values were (109.2±7.5), (83.6±6.0), and (54.3±2.8) μmol/L respectively (P0.01). After CNE-2Z cells were treated with 25, 50, 100, and 200 μmol/L Res for 24 h, the apoptotic rates by both flow cytometry and fluorescence staining at 50, 100 and 200 μmol/L Res-treated groups were significantly higher than those in control groups (P0.01). The typical morphological changes in apoptosis were found by fluorescence staining. The results of DNA agarose gel electrophoresis showed that DNA ladder appeared obviously at 100 and 200 μmol/L Res-treated groups. Conclusion Res can inhibit the proliferation of cell line CNE-2Z through the induction of apoptosis.