Effects of Resveratrol on Proliferation of MCF-7 Cells in Breast Cancer

Objective:Our goals were to examine the phytoestrogen-like effect of Resveratrol(RES) using in vitro tests.Methods:Effects of RES on proliferation of MCF-7 cells were detected by MTT test.The fluorescent microscopes by the staining of Hoechst 33342 and DNA gel electrophoresis analysis were used to determine the effects of MCF-7 cells by RES.Immunohistochemistry was used to assess PCNA expression.Results:A MTT test and growth curve showed that the proliferation of MCF-7 cells was inhibited by a high concentration of RES,and that its IC50 was 8.70×10-5mol/L.However,RES induced the proliferation of MCF-7 cells at 10-7~10-6mol/L,and resulted in a peak proliferation at 1.0×10-7mol/L.After the cells were treated with high concentrations RES,morphological characteristic of apoptosis was detected.Agarose gel electrophoresis showed typical DNA ladder.The expression of PCNA was increased after treated with low concentrations of RES.Conclusions:These results suggest that RES had a dual regulatory effect on MCF-7 cells.