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Studies on Heterotic Group of Middle Early Mature Maize Inbred Lines in Heilongjiang by SSR Markers

Xu Chongxiang, Min Li, An Yinghui, Weizhong Li, Mei Sun

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Abstract

The genetic diversity of 25 middle early mature maize inbred lines in Heilongjiang was analyzed by SSRs,and the heterotic groups were classified.Eighty pairs of primers whose bands were clearly amplified and discrepant were selected out of 100.Totally 361 alleles were identified,and the average number of alleles per SSR locus was 4.513 in these lines with a range from 2 to 8.Polymorphism information content(PIC) values varied from 0.298 to 0.827 with an average of 0.615.The genetic similarity coefficient(GS) ranged from 0.554 to 0.939 among 25 inbred lines.By clustering analysis,25 inbred lines were divided into 4 heterotic groups.Cluster analyses by UPGMA were basically corresponding to their pedigree analysis.

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The genetic diversity of 25 middle early mature maize inbred lines in Heilongjiang was analyzed by SSRs,and the heterotic groups were classified.Eighty pairs of primers whose bands were clearly amplified and discrepant were selected out of 100.Totally 361 alleles were identified,and the average number of alleles per SSR locus was 4.513 in these lines with a range from 2 to 8.Polymorphism information content(PIC) values varied from 0.298 to 0.827 with an average of 0.615.The genetic similarity coefficient(GS) ranged from 0.554 to 0.939 among 25 inbred lines.By clustering analysis,25 inbred lines were divided into 4 heterotic groups.Cluster analyses by UPGMA were basically corresponding to their pedigree analysis.

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Available abstract

The genetic diversity of 25 middle early mature maize inbred lines in Heilongjiang was analyzed by SSRs,and the heterotic groups were classified.Eighty pairs of primers whose bands were clearly amplified and discrepant were selected out of 100.Totally 361 alleles were identified,and the average number of alleles per SSR locus was 4.513 in these lines with a range from 2 to 8.Polymorphism information content(PIC) values varied from 0.298 to 0.827 with an average of 0.615.The genetic similarity coefficient(GS) ranged from 0.554 to 0.939 among 25 inbred lines.By clustering analysis,25 inbred lines were divided into 4 heterotic groups.Cluster analyses by UPGMA were basically corresponding to their pedigree analysis.

Key concepts: UPGMA, Inbred strain, Locus (genetics), Biology, Genetic diversity, Genetic similarity, Genetics, Allele

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