Analysis of Genetic Diversity of 48 Local Maize Inbred Lines by SSR Markers

The genetic diversity of 48 maize inbred lines in Heilongjiang was analyzed by SSRs and the heterotic groups were classified.20 pairs of primers whose bands were clearly amplified and discrepant were selected out from 100 pairs of primers.Totally 105 alleles were identified,and the average number of alleles per SSR locus was 5.25 in these lines with a range from 3 to 7.Polymorphism information content(PIC) values varied from 0.405 to 0.793 with an average of 0.664.The genetic similarity coefficient(GS) ranged from 0.400 to 0.905 among 48 inbred lines.By clustering analysis,48 inbred lines were divided into 5 heterotic groups.Cluster analysis by UPGMA were basically corresponding to their pedigree analysis.Therefore,SSR could be used to divide heterotic groups.